|
Source:
New York
Times, February 13, 2002.
TRACKING THE DISEASE
Scientist's Findings Could
Aid Anthrax Inquiry
By NICHOLAS WADE
LAS VEGAS, Feb. 12 -- In what could provide a major
break in the hunt for the sender of anthrax-laden letters last fall, a
researcher studying the case for the F.B.I. says he has distinguished between
stocks of the anthrax strain kept in different laboratories.
The method should help tell which
laboratory's stock of anthrax is closest to that used by the attacker. That
could narrow the search to people with access to that particular laboratory and
its stock of anthrax.
The researcher,
Dr. Paul Keim of
Northern Arizona University, made the announcement here at a national conference
on microbial genomes.
Asked which laboratories had provided
the stocks he studied, Dr. Keim said his agreement with the Federal Bureau of
Investigation prevented him from discussing the case. The F.B.I., which refused
to comment on his remarks, has said it has no firm suspects, but it has focused
on insiders who may have had access to anthrax in laboratories.
Many strains of anthrax exist, but the
one used in the attacks is called Ames, first isolated from a cow in Texas in
1981. Because of the strain's virulence, it was studied for years by the Army's
laboratory for biological warfare defense at Fort Detrick, Md., and was
distributed to several laboratories in the United States and abroad to help them
test vaccines.
After the anthrax attacks, researchers
tried to discriminate between the various stocks of Ames to see whether they
could pinpoint the laboratory of origin. But since all the stocks came from a
single source, the bacteria were essentially members of a single large clone, as
alike genetically as identical twins.
A DNA fingerprinting test for anthrax
bacteria, similar to the test used on humans in criminal cases, had been
developed by Dr. Keim and colleagues.
Dr. Keim's fingerprinting test, which
was based on eight points of difference, could not distinguish between the
different stocks of Ames anthrax, and he set about trying to develop more
markers, which are sites on the DNA at which some anthrax bacteria have a
different sequence of DNA letters from other bacteria.
To help in the search for new markers,
the National Science Foundation asked the Institute for Genomic Research in
Rockville, Md., to decode the full DNA sequence of the anthrax bacteria
recovered from Robert
Stevens, the photo editor who died in the Florida attack. The institute was
already sequencing the full genome of the Ames strain owned by the Fort Detrick
laboratory, so it would be in a position to look for DNA differences throughout
the bacterium's genome.
The institute focused on the main
chromosome of the bacteria, a large ring of DNA now known to contain 5,167,515
DNA letters holding information for 5,960 genes. The bacterium also contains two
small rings of DNA known as plasmids, which carry the genes essential for its
virulence. The plasmid's DNA was decoded several years ago by scientists at the
Los Alamos Nuclear Laboratory.
Dr. Keim's success came from studying a
site on the second of these plasmids called a poly-A tract. He found that Ames
stocks held in different laboratories varied in the number of A's -- one of the
four units of DNA -- they contained in the poly-A tract. The number of A's
varied from 8 to 25, the exact number depending on the laboratory that provided
the stock.
On the basis of the poly-A test, he
said, he has been able to distinguish between the Ames strains of anthrax held
in four laboratories, and in a natural Ames isolate taken from a goat in 1997.
Because of his agreement with the F.B.I., Dr. Keim would not name the
laboratories or say from how many other laboratories he had received samples.
But another anthrax expert at the
meeting said that if Dr. Keim had samples from all laboratories having the Ames
strain, he should be able to say which one the attack strain most resembled and
might have already done so. |